Specificity for Nicotinamide Adenine Dinucleotide and Nicotinamide Adenine Dinucleotide Phosphate of Nitrate Reductase from the Salt-tolerant Alga Dunaliella parva

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Specificity for Nicotinamide Adenine Dinucleotide and Nicotinamide Adenine Dinucleotide Phosphate of Nitrate Reductase from the Salt-tolerant Alga Dunaliella parva.

Nitrate reductase of the salt-tolerant alga Dunaliella parva could utilize NADPH as well as NADH as an electron donor. The two pyridine nucleotide-dependent activities could not be separated by either ion exchange chromatography on DEAE-cellulose or gel filtration on Sepharose 4B. The NADPH-dependent activity was not inhibited by phosphatase inhibitors. NADPH was not hydrolyzed to NADH and inor...

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Specificity for nicotinamide adenine dinucleotide by nitrate reductase from leaves.

Preliminary work revealed that nitrate reductase in crude extracts prepared from leaves of certain corn genotypes as well as soybeans could utilize NADPH as well as NADH as the electron donor. Isoelectric focusing and diethylaminoethyl cellulose chromatography confirmed previous findings that NADH and NADPH activities could not be separated, which suggests the involvement of a single enzyme. Ni...

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Measurement of nicotinamide adenine dinucleotide & nicotinamide adenine dinucleotide phosphate in tomato leaves.

As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. Thes...

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Inhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.

Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...

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Nicotinamide Adenine Dinucleotide Phosphate - specific Isocitrate

Nicotiniamide adenine dintucleotide plhosphate-specific isocitrate dehydrogenase was extracted from etiolated pea (Pisurn satirum L.) seedlings and was purified 65-fold. The puirified enzyme exhibits one predoniinant protein band by pol acrylamide gel electrophoresis, w-hich corresponds to tiie dehy-drogenase activity as imieasuired by the nitro bluie tetrazolium technique. The reaction is read...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1976

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.58.1.57